Monoclonal antibodies against enterocytozoon bieneusi of human origin

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dc.creatorSheoran, Abhineet S.
dc.creatorFeng, Xiaochuan
dc.creatorSingh, Inderpal
dc.creatorChapman-Bonofiglio, Susan
dc.creatorKitaka, Sabrina
dc.creatorHanawalt, Joel
dc.creatorNunnari, John
dc.creatorMansfield, Keith
dc.creatorTumwine, James K.
dc.creatorTzipori, Saul
dc.date2012-05-25T08:39:53Z
dc.date2012-05-25T08:39:53Z
dc.date2005
dc.date.accessioned2018-09-04T12:32:38Z
dc.date.available2018-09-04T12:32:38Z
dc.descriptionEnterocytozoon bieneusi is clinically the most significant among the microsporidia infecting humans, causing chronic diarrhea, wasting, and cholangitis in individuals with human immunodeficiency virus/AIDS. The lack of immune reagents is largely due to the absence of methods for laboratory propagation of E. bieneusi. We recently described a procedure for the concentration and purification of spores from diarrheic stool of infected humans. Purified spores were used to immunize mice for production and screening of monoclonal antibodies (MAbs) against E. bieneusi. The eight immunoglobulin M MAbs generated and fully characterized did not cross-react with other human microsporidia or with other microorganisms normally present in stool. One of the MAbs, 2G4, reacted with E. bieneusi spores in stools from monkeys and humans, without background fluorescence, which makes it an ideal diagnostic reagent. It also recognizes intracellular stages of the parasite and will be suitable for determining tissue distribution of E. bieneusi in infected hosts. At least two immunodominant antigens of E. bieneusi of 33,000 and 35,000 Da exist, which were recognized by rabbit and mouse antisera. The availability of MAbs against E. bieneusi will simplify considerably the diagnosis of this infection in humans and will provide tools for epidemiologic investigations regarding the true prevalence of the infection in various human and mammalian populations and the environmental sources of infection
dc.identifierSheoran, A. S., et al. (2005). Monoclonal antibodies against Enterocytozoon bieneusi of human origin. Clinical and Diagnostic Laboratory Immunology, 12(9):1109-1113
dc.identifier1071-412X
dc.identifierhttp://dx.doi.org/10.1128/CDLI.12.9.1109-1113.2005
dc.identifierhttp://hdl.handle.net/10570/572
dc.identifier.urihttp://hdl.handle.net/10570/572
dc.languageen
dc.publisherAmerican Society for Microbiology
dc.relationClinical and Diagnostic Laboratory Immunology;12(9)
dc.subjectMonoclonal antibodies
dc.subjectEnterocytozoon bieneusi
dc.subjectEnteric protozoon
dc.subjectE. bieneusi
dc.subjectMicrosporidia
dc.titleMonoclonal antibodies against enterocytozoon bieneusi of human origin
dc.typeJournal article, peer reviewed
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