Comparison of methods in the recovery of nucleic acids from archival formalin-fixed paraffin-embedded autopsy tissues

Simple item page
dc.creatorOkello, John B.A.
dc.creatorZurek, Jaymi
dc.creatorDevault, Alison M.
dc.creatorKuch, Melanie
dc.creatorOkwi, Andrew L.
dc.creatorSewankambo, Nelson K.
dc.creatorBimenya, Gabriel S.
dc.creatorPoinar, Debi
dc.creatorPoinar, Hendrik N.
dc.date2011-12-15T16:01:37Z
dc.date2011-12-15T16:01:37Z
dc.date2010-05
dc.date.accessioned2018-09-04T12:53:01Z
dc.date.available2018-09-04T12:53:01Z
dc.descriptionArchival formalin-fixed paraffin-embedded (FFPE) human tissue collections are typically in poor states of storage across the developing world. With advances in biomolecular techniques, these extraordinary and virtually untapped resources have become an essential part of retrospective epidemiological studies. To successfully use such tissues in genomic studies, scientists require high nucleic acid yields and purity. In spite of the increasing number of FFPE tissue kits available, few studies have analyzed their applicability in recovering high-quality nucleic acids from archived human autopsy samples. Here we provide a study involving 10 major extraction methods used to isolate total nucleic acid from FFPE tissues ranging in age from 3 to 13 years. Although all 10 methods recovered quantifiable amounts of DNA, only 6 recovered quantifiable RNA, varying considerably and generally yielding lower DNA concentrations. Overall, we show quantitatively that TrimGen’s WaxFree method and our in-house phenol–chloroform extraction method recovered the highest yields of amplifiable DNA, with considerable polymerase chain reaction (PCR) inhibition, whereas Ambion’s RecoverAll method recovered the most amplifiable RNA.
dc.identifierOkello, J.B.A., Zurek, J., Devault, A.M., Kuch, M., Okwi, A.L., Sewankambo, N.K., Bimenya, G.S., Poinar, D., Poinar, H.N. (2000). Comparison of methods in the recovery of nucleic acids from archival formalin-fixed paraffin-embedded autopsy tissues. Analytical Biochemistry, 400
dc.identifierhttp://hdl.handle.net/10570/253
dc.identifier.urihttp://hdl.handle.net/10570/253
dc.languageen
dc.publisherElsevier
dc.subjectArchival FFPE tissues
dc.subjectDNA/RNA extraction
dc.subjectHistopathology
dc.subjectInhibition
dc.subjectNucleic acid
dc.subjectQuantitative PCR
dc.titleComparison of methods in the recovery of nucleic acids from archival formalin-fixed paraffin-embedded autopsy tissues
dc.typeJournal article, peer reviewed
Files
Collections
Dissertation and Thesis