Complexity of plasmodium falciparum clinical samples from Uganda during short-term culture.

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dc.creatorNsobya, Samuel L.
dc.creatorKiggundu, Moses
dc.creatorJoloba, Moses
dc.creatorDorsey, Grant
dc.creatorRosentha, Philip J.
dc.date2011-12-15T16:17:59Z
dc.date2011-12-15T16:17:59Z
dc.date2008-08-22
dc.date.accessioned2018-09-04T12:53:03Z
dc.date.available2018-09-04T12:53:03Z
dc.descriptionWe cultured Plasmodium falciparum parasites from 98 Ugandan children with malaria and determined the complexity of infection (COI) on the basis of msp-2 polymorphisms daily for 9 days. The mean COI decreased during culture from 1.73 to 1.56. New strains appeared after day 0 in 20 cultures. Strains disappeared after day 0 in56%of 45 cultures that were initially mixed; persisting strains more commonly had wild-type dhfr (C59) and dhps (K540) sequences and mutant pfmdr1 (86Y) sequences. Thus, initial genotypes offer an imperfect representation of clinical COI. Loss of strains in culture may be due to diminished fitness of some drug-resistant strains.
dc.identifierNsobya, S.L., Kiggundu, M., Joloba, M., Rosenthal, P.J., Dorsey, G. (2008). Complexity of plasmodium falciparum clinical samples from Uganda during short-term culture. Journal of Infectious Diseases, 198
dc.identifier0022-1899
dc.identifierhttp://dx.doi.org/10.1086/592506
dc.identifierhttp://hdl.handle.net/10570/254
dc.identifier.urihttp://hdl.handle.net/10570/254
dc.languageen
dc.publisherUniversity of Chicago Press
dc.subjectUganda
dc.subjectMalaria
dc.subjectGenotypes
dc.subjectMalaria parasites
dc.subjectPlasmodium falciparum
dc.subjectChildren
dc.subjectAntimalarial therapy
dc.titleComplexity of plasmodium falciparum clinical samples from Uganda during short-term culture.
dc.typeJournal article, peer reviewed
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